Page 67 - MaquetaFundCIEN-2019-ENG

• The Vallecas Project
It is currently known that the pathological processes that determine Alzheimer begin many years before the disease leads to the first noticeable symptoms in patients. Years before that future drug treatments preventing or slowing down dis- ease progression could be applied to the “population at risk” who has developed these subclinical lesions, or has a higher risk of developing it than the rest of the population. In this context it is framed the Vallecas Project, which is constituted as a 5-year longitudinal study specifically aimed at discovering the factors that would allow us to detect this “population at risk” in a phase of potentially treata- ble pathology.
During 2019, 300 volunteers were studied, with the following distribution: 1 volun- teer from the fifth follow-up visit of the study, 33 volunteers from the sixth visit, 142 from the seventh visit and 124 from the eighth visit.
Of all volunteers recruited in the study and having an informed consent, a blood sample is collected and immediately transferred to the laboratory for fractiona- tion into aliquots following the so-called Vienna Institute of Neurology protocol, which allow different types of analysis, as well as classification and storage (see Figure below). Additionally, one blood tube (BD-CPT citrate Vacutainer) for the isolation of mononuclear leukocytes, together with another tube lacking antico- agulant to obtain serum are processed.
Vienna Institute of Neurology Protocol for blood processing in various fractions for the search for biomarkers and susceptibility genes.
Within the Molecular Genetics department, the activity of the Vallecas Project is shown in the table on the following page:
3. Scientific activity
67
EVALUATION
1ST
2ND
3RD
4TH
5TH
6TH
7TH
8TH
TOTAL
No SAMPLES
1169
767
755
699
662
414
172
7
4645
2019 SAMPLES
-
-
-
-
1
33
142
124
300
TOTAL
1169
767
755
699
663
447
314
131
4945
No ALIQUOTS
16366
10738
10570
9786
9282
6258
4396
1834
69230
The type of primary aliquots that are obtained in duplicate are the following:
• Whole blood (ST, for its acronym in Spanish) • Platelets-rich plasma (PRP)
• Platelets-free plasma (PFP)
• Buffy Coat (BC)
• Red blood cells (RBC)
• Serum (Suero, in Spanish)
• Mononucleate leukocytes (LM, for its acronym in Spanish)