Page 86 - MaquetaFundCIEN-2019-ENG

86
Annual Report 2019
ples will be collected within the Vallecas Project for the study of genetic and bi- ochemical markers. Samples are obtained according to the protocol “Collection and Processing of Human Blood Samples in the Vallecas Project” and processed to obtain the various fractions indicated in the protocol, which are stored at -80 . On one hand, DNA is extracted from blood cells to determine, by PCR and sequencing techniques, genetic markers associated with the various poly- morphisms in the following genes: APOE, CR1, BIN1, CLU, PICALM, ABCA7, SORL1, PRNP, GRM8, and BACE1. These genes are studied using DNA obtained from the extraction of samples from the first visit.
Also, in the context of the project funded by the MINECO (Projects RETOS) en- titled “miRNA and lipid metabolism markers as potential links between vascular dysfunction and Alzheimer’s pathophysiology”, and whose main researchers are Drs. Miguel Medina and Miguel Calero, in collaboration with the group of Dr. To- bias Engel (Royal College of Surgeons, Dublin, Ireland) plasma-derived microR- NAs are being analyzed as potential biomarkers, as well as molecules related to vascular dysfunction, lipid metabolism and inflammation: Adiponectin/Acrp30, P-Selectin, ICAM-1, IL-6, MMP-9, Serpin E1/PAI-1, TNF-alpha, VCAM-1, CCL2/MCP- 1, IL-1 beta, CXCL8/IL-8, E-Selectin, MMP-3, and CRP. The usefulness of these biomarkers is complementary with the information derived from the study of genetic risk markers already mentioned and can define risk factors already re- vealed in previous studies.
Samples collected and processed to date are summarized in the table below:
EVALUATION
TOTAL
1st Visit
1169
2nd Visit
767
3rd Visit
755
4th Visit
699
5th Visit
663
6th Visit
447
7th Visit
314
8th Visit
131
TOTAL
4945