Page 59 - Memora anual Fundación CIEN 2015
P. 59
3. SCIENTIFIC ACTIVITY
Evaluation The Vallecas Project activity in figures TOTAL
1º 2º 3º 4º 5º
Samples 1.169 829 577 15 0 2.633
2015 samples 0 22 245 441 5 757
Total 1.169 851 822 456 5 3.390
Aliquots 16.366 11.914 11.508 6.384 70 47.460
Vienna Institute of Neurology Protocol for blood processing
in various fractions for the search for biomarkers and susceptibility genes
FIGURE 1 10ml Whole Blood
2x0.5 ml backup aliquot 9 ml centrifugation (2280g)
( -80ºC) WHO proposal Institute of Neurology Vienna
~ 4ml PRP 2x1.3 ml aliquots ( -80ºC)
1.3 ml centrifugation (14000 rpm)
PRP: platelet-rich plasma ~ 1ml BC 1 ml aliquot platelet-free plasma
BC: buffy coat ~ 4 ML RBC ( -80ºC)
RBC: red blood cells
2x 0.25 ml aliquots (WHO)
( -80ºC)
2x 0.25 ml aliquots (genotyping)
( -80ºC, separately)
2x 1.7 ml aliquots ( -80ºC)
CIEN Foundation Annual Report 2015 / 61
