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The type of primary aliquots that are obtained in It is also important to emphasize that the samples ob-
duplicate are the following: tained from Vallecas Project volunteers aged bet-
ween 70 and 85 years (at the baseline evaluation)
• Whole blood (ST, for its acronym in Spanish) that include a comprehensive assessment of cogni-
• Platelets-rich plasma (PRP) tive, sociological and neuroimaging state are opti-
• Platelets-free plasma (PFP) mal for its use as a control population in various
• Buffy Coat (BC) projects related to neurodegenerative diseases, es-
• Red blood cells (RBC) pecially Alzheimer's disease. The monitoring for a pe-
• Serum (Suero, in Spanish) riod of 5 years will allow us to detect early, even
• Mononucleate leukocytes (LM, for its before clinical symptoms manifestation, susceptibi-
lity factors and biomarkers associated with Alzhei-
acronym in Spanish) mer's disease.
Genomic DNA was extracted from whole blood of Research Program of the Queen Sofia Foundation
all participants who have signed informed consent Alzheimer Center, This program focuses on regular
to it and the APOE gene, an important marker of ge- and protocol-based monitoring of a cohort of CAFRS
netic risk for Alzheimer's disease, was analyzed. The patients with dementia, ether as residents at the
comparison of the frequency of APOE allele 4 ca- Center or attendants at the Day Center, with the
rriers between CAFRS patients and Vallecas Project main objective of investigating the final stages of Alz-
volunteers confirms the risk to suffer from Alzheimer's heimer's disease. A family member or guardian re-
disease with an OR = 3.53 (p <0.001). In addition, in cruits patients into the monitoring program after
order to define different subpopulations of genetic signing an Informed Consent. The Alzheimer Project
risk, other possible genetic susceptibility genes have program consists of i) a biannual clinical and neu-
also been analyzed in a subset of participants (see fi-
gure).
The Vallecas Project activity in figures
Evaluation 1º 2º 3º 4º 5º 6º TOTAL
Total samples 1.169 767 755 648 384 0 3.723
2017 samples 0 00 51 234 249 534
Total 1.169 767 755 699 618 249 4.257
Nº Aliquotes 16.366 10.738 10.570 9.786 8.652 3.486 59.598
CIEN Foundation Annual Report 2017 / 78
