Page 77 - Memora anual Fundación CIEN 2017

3. SCIENTIFIC ACTIVITY

The phase of recruiting volunteers for participation in tionation into aliquots following the so-called Vienna
the study was finished in December 31, 2013, with its Institute of Neurology protocol, which allow different
corresponding baseline assessment (n=1,169). The types of analysis, as well as classification and storage
project includes activities from the Neurology, Neu- (see Figure below). Additionally, one blood tube
ropsychology, Neuroimaging, and Molecular Gene- (BD-CPT citrate Vacutainer) for the isolation of mo-
tics departments. During 2017, we have studied 51 nonuclear leukocytes, together with another tube
volunteers to complete the fourth follow-up visit, 234 lacking anticoagulant to obtain serum are proces-
volunteers on the fifth visit, and 249 on the sixth visit. sed..

Of all patients recruited in the study and having an Within the laboratory department, the activity of the
informed consent, a blood sample is collected and Vallecas Project is shown in the table on the follo-
immediately transferred to the laboratory for frac- wing page.

Vienna Institute of Neurology Protocol for blood processing
in various fractions for the search for biomarkers and susceptibility genes

10ml Whole Blood Blood without anticoagulant

2x0.5 ml backup aliquot 9 ml centrifugation (2280g) Serum
Platelet-rich plasma
( -80ºC) WHO proposal Institute of Neurology Vienna

~ 4ml PRP 2x1.3 ml aliquots ( -80ºC)

Whole blood 1.3 ml centrifugation (14000 rpm) Plasma
(anti-coagulated) Buffy coat
1 ml aliquot platelet-free plasma
~ 1ml BC ( -80ºC) DNA

2x 0.25 ml aliquots (WHO)
( -80ºC)

2x 0.25 ml aliquots (genotyping)
( -80ºC, separately)

~ 4 ML RBC 2x 1.7 ml aliquots ( -80ºC) Red blood cells

PRP: platelet-rich plasma Modifed proposal for collection, separation and storage of Blond
BC: buffy coat and Blood components from TSE infected humans, Institute of
RBC: red blood cells Neurology, University of Viena, May 2001. Modified proposal
blood collection WHO 080501).

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