Page 59 - Annual Report 2018

3. SCIENTIFIC ACTIVITY

The phase of recruiting volunteers for participation in Of all patients recruited in the study and having an
the study was finished in December 31, 2013, with its informed consent, a blood sample is collected and
corresponding baseline assessment (n=1,169). The immediately transferred to the laboratory for frac-
project includes activities from the five research tionation into aliquots following the so-called Vienna
areas of the CIEN Foundation. During 2018, 388 vo- Institute of Neurology protocol, which allow different
lunteers were studied, distributed in 44 volunteers types of analysis, as well as classification and storage
from the fifth follow-up visit of the study, 165 volun- (see Figure below). Additionally, one blood tube
teers from the sixth visit, 172 from the seventh visit (BD-CPT citrate Vacutainer) for the isolation of mo-
and 7 from the eighth visit. nonuclear leukocytes, together with another tube

Protocol for blood fraction collection, separation and storing

10ml Whole Blood
Blood without anticoagulant

Serum
2x0.5 ml backup aliquot 9 ml centrifugation (2280g)
( -80ºC)
WHO proposal Institute of Neurology Vienna
~ 4ml PRP 2x1.3 ml aliquots ( -80ºC) Patelet-rich plasma

1.3 ml centrifugation (14000 rpm)
Whole Blood
(anti-coagulated)
1 ml aliquot platelet-free plasma Plasma
( -80ºC)
~ 1ml BC 2x 0.25 ml aliquots (WHO)
( -80ºC) Buffy coat
2x 0.25 ml aliquots (genotyping)
( -80ºC, separately) DNA
~ 4 ML RBC 2x 1.7 ml aliquots ( -80ºC) Red blood cells

Modifed proposal for collection, separation and storage of Blond
PRP: platelet-rich plasma and Blood components from TSE infected humans, Institute of
BC: buffy coat Neurology, University of Viena, May 2001. Modified proposal
RBC: red blood cells
blood collection (WHO 080501).
Vienna Institute of Neurology Protocol for blood processing in various fractions for the
search for biomarkers and susceptibility genes.

CIEN Foundation Annual Report 2018 / 59